Extracellular pullulanase synthesis inBacillus macerans
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منابع مشابه
Pullulanase from rice endosperm.
Pullulanase (EC 3.2.1.41) in non-germinating seeds was compared with that in germinating seeds. Moreover, pullulanase from the endosperm of rice (Oryza sativa L., cv. Hinohikari) seeds was isolated and its properties investigated. The pI value of pullulanase from seeds after 8 days of germination was almost equal to that from non-germinating seeds, which shows that these two enzymes are the sam...
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The extracellular enzymatic activity of a mixed culture of anaerobic marine bacteria enriched on pullulan [alpha(1,6)-linked maltotriose units] was directly assessed with a combination of gel permeation chromatography (GPC) and nuclear magnetic resonance spectroscopy (NMR). Hydrolysis products of pullulan were separated by GPC into three fractions with molecular weights of > or = 10,000, approx...
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2-Phenylacetamide is an effective germinant for spores of five strains of Bacillus macerans, particularly in the presence of fructose. Benzyl penicillin, the phenyl acetamide derivative of penicillin, and phenylacetic acid are also good germinants. l-Asparagine is an excellent germinant for four strains. alpha-Amino-butyric acid is moderately effective. Pyridoxine, pyridoxal, adenine, and 2,6-d...
متن کاملSome Properties of a Pullulanase by Gwen
1. A pullulanase has been separated from cell extracts ofStreptococcus mitis. The enzyme was freed from transglucosylase by fractionation with ammonium sulphate. 2. Pullulanase was produced in the absence of inducers, and addition of glucose or maltose to the broth did not increase the yield of enzyme. 3. The pullulanase'acted rapidly on a-(1-+6)-bonds in substrates having the structure a-malto...
متن کاملPullulanase synthesis in klebsiella (aerobacter) aerogenes strains growing in continuous culture.
1. Pullulanase synthesis was studied in 16 classified (N.C.I.B.) strains and in an industrial strain (R) of Klebsiella aerogenes grown in chemostats containing maltose as inducer and sole carbon source. 2. Maximum synthesis was associated with carbon-limited growth at a low dilution rate (about 0.2h(-1)). The enzyme remained firmly cell-bound and seemed to be located on the cell surface. 3. Thr...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1977
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1977.tb00630.x